Fig. 1
A high-fat diet inhibits fear extinction in male mice. (A) Experimental timeline used to study the effect of diet and repeated stress on mice. (B) Percent freezing on day 2 of SEFL (n = 20/19; two-tailed unpaired t test, t = 1.913; p = 0.0635). (C) Shock reactivity measured as velocity (cm/s) on day 2 (n = 20/19; two-tailed unpaired t test, t = 1.875; p = 0.0687). (D) Percent freezing on day 3 of SEFL (n = 20/19; two-tailed unpaired t test, t = 1.814; p = 0.3916). (E) Percent freezing of HFD-fed male mice during 14 weeks of RS/NRS treatment (n = 4,5; two-way ANOVA, F1,98 =69.18; ****p < 0.0001; post hoc comparison, n = 5/4; Sidak’s multiple comparisons test, p > 0.99). (F) The freezing percentage of HFD-fed male mice during the freezing test conducted after 14 weeks (n = 5/4, two-tailed paired t test; t = 2.518, p = 0.08). (G) Percent freezing of HFD female mice during 14 weeks of RS/NRS treatment (n = 5; two-way ANOVA, F1,112 =157.1; ****p < 0.0001) (post hoc comparison, n = 5; Sidak’s multiple comparisons test, **p < 0.01). (H) Percent freezing in HFD females in the freezing test after 14 weeks (n = 5, two-tailed paired t test; t = 3.514, *p < 0.05)