Fig. 5

Repeated reminder shocks and a high-fat diet (HFD) induced distinctive alterations in peripheral myeloid lineage cells and inflammatory markers, exhibiting a sexually dimorphic pattern. (A) Quantification of the bone marrow population of MPP2 progenitors in HFD-fed mice (n = 9; two-way ANOVA, F1,14 = 290.1; ****p < 0.0001) (post hoc comparison, n = 5; Tukey’s multiple comparison test, *p < 0.05 and ****p < 0.0001). (B) Quantification of the MPP3 progenitor population in the bone marrow of HFD-fed mice (n = 9; two-way ANOVA; F1,14 = 34.48; ****p < 0.0001) (post hoc comparison, n = 5; Tukey’s multiple comparison test, p = 0.08 and ***p < 0.001). (C) Quantification of granulocyte monocyte progenitor (GMP) cells in HFD-fed mice (n = 9; two-way ANOVA, F1,14 = 42.52; ****p < 0.0001) (post hoc comparison, N = 4/5; Tukey’s multiple comparison test, **p < 0.01). (D) Quantification of monocyte-dendritic cell progenitors (MDPs) in the bone marrow of HFD-fed mice (n = 9; two-way ANOVA, F1,14 = 6.017, *p < 0.05) (post hoc comparison, n = 5; Tukey’s multiple comparison test, *p < 0.05). (E) Quantification of neutrophils in the blood of HFD-fed mice (n = 9; two-way ANOVA, p > 0.05). (F) Quantification of Ly6Chi monocytes in the blood of HFD-fed mice (n = 9; two-way ANOVA, p > 0.05). (G) Quantification of neutrophils in the gonadal white adipose tissue (gWAT) of HFD-fed mice (n = 9; two-way ANOVA, F1,14 = 24.65, ***p < 0.001) (post hoc comparison, n = 5; Tukey’s multiple comparison test, *p < 0.05 and **p < 0.01). (H) Quantification of macrophages in the gWAT of HFD-fed mice (n = 9; two-way ANOVA; F1,14 = 6.296; *p < 0.05) (post hoc comparison; n = 4/5; Tukey’s multiple comparison test; *p < 0.05). (I) Quantification of aortic neutrophils from HFD-fed mice (n = 9; two-way ANOVA; F1,14 = 59.59; ****p < 0.0001) (post hoc comparison; n = 4/5; Tukey’s multiple comparison test; ***p < 0.001). (J) Quantification of macrophages in the aortas of HFD-fed mice (n = 4/5; Tukey’s multiple comparison test, p > 0.05). K. Quantification of neutrophils in the hearts of HFD-fed mice (n = 9; two-way ANOVA, p > 0.05). L. Quantification of macrophages in the hearts of HFD-fed mice (n = 9; two-way ANOVA; F1,14 = 32.04; ****p < 0.0001) (post hoc comparison; n = 4/5; Tukey’s multiple comparison test; *p < 0.05 and **p < 0.01)