Fig. 3

Lipid subclasses most affected by UGT KO. A. Schematic pathway of cholesterol-derived progestogens and androgens. Steroids that differ significantly in at least one UGT KO group are shown as tile arrays depicting sense of fold change relative to the reference group (blue, lower; red, higher). Most steroids that were measured were sulfate (-S) or glucuronide (-G) conjugates. Each asterisk denotes that the steroid has two sulfate groups. O: overall; M: males; F: females. B. Simplified sphingolipid metabolic pathway and sphingolipids significantly altered in UGT2B17 KO overall analysis. The global sense of the fold change for each sphingolipid subclass is indicated by color (blue, lower; light red, higher). The numbers of measured (center) and altered sphingolipids (parts of the donut) are given for each subclass. DCER, dihydroceramides; DhSM, dihydrosphingomyelins; CER, ceramides; HCER, hexosylceramides; LCER, lactosylceramides; SM, sphingomyelins. C. Phospholipid profiles in UGT2B17 KO and UGT2B28 KO groups relative to reference. LPC, lysophosphatidylcholines; LPE, lysophosphatidylethanolamines; LPI, lysophosphatidylinositol; LPG, lysophosphatidylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol. *P < 0.05. (D) Fatty acid and (E) acylcarnitine profiles for UGT2B28 KO females relative to the reference group. Fold changes are shown relative to the reference group level set at 1.0 (dashed line). The x axis indicates carbon chain length and includes fatty-acid dicarboxylates (DC), monohydroxy fatty acids or hydroxy acyl carnitines (2OH, 3OH, 16OH), and branched-chain fatty acids (CH3). 3-CMPFP, 3-carboxy-4-methyl-5-pentyl-2-furanpropionate; CAR, carnitine. The terms 22:3* and 22:5* denote omega-6 polyunsaturated fatty acids